Context: will be measuring the absorbance of para-nitrophenol in a buffer solution with pH 6.3.

Estimate the absorbance (“extinction”) coefficient. For 100% anion the value is 1.86 x 10^4. Assume the pKa is 7. Calculate the value of the coefficient at pH6.3. (What fraction of the pNP will be the anion at this pH?)

Context: will be measuring the absorbance of para-nitrophenol in a buffer solution with pH 6.3.

Estimate the absorbance (“extinction”) coefficient. For 100% anion the value is 1.86 x 10^4. Assume the pKa is 7. Calculate the value of the coefficient at pH6.3. (What fraction of the pNP will be the anion at this pH?)

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