Context: will be measuring the absorbance of para-nitrophenol in a buffer solution with pH 6.3. Estimate the absorbance (“extinction”) coefficient. For 100% anion the value is 1.86 x 10^4. Assume th


Context: will be measuring the absorbance of para-nitrophenol in a buffer solution with pH 6.3.

Estimate the absorbance (“extinction”) coefficient.  For 100% anion the value is 1.86 x 10^4.  Assume the pKa is 7.  Calculate the value of the coefficient at pH6.3.  (What fraction of the pNP will be the anion at this pH?)

Context: will be measuring the absorbance of para-nitrophenol in a buffer solution with pH 6.3. Estimate the absorbance (“extinction”) coefficient. For 100% anion the value is 1.86 x 10^4. Assume th


Context: will be measuring the absorbance of para-nitrophenol in a buffer solution with pH 6.3.

Estimate the absorbance (“extinction”) coefficient.  For 100% anion the value is 1.86 x 10^4.  Assume the pKa is 7.  Calculate the value of the coefficient at pH6.3.  (What fraction of the pNP will be the anion at this pH?)

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